mruby2 c1 Search Results


mruby2 c1 addgene  (Addgene inc)
92
Addgene inc mruby2 c1 addgene
Mruby2 C1 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2 c1 addgene/product/Addgene inc
Average 92 stars, based on 1 article reviews
mruby2 c1 addgene - by Bioz Stars, 2026-03
92/100 stars
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mruby2  (Addgene inc)
92
Addgene inc mruby2
(A) Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with <t>mAID/mRuby2.</t> (B) Order of events for the generation of the different cell lines. (C) Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. (D) Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. (E) Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. Tukey HSD test: *p < 0.05.
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2/product/Addgene inc
Average 92 stars, based on 1 article reviews
mruby2 - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


(A) Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. (B) Order of events for the generation of the different cell lines. (C) Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. (D) Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. (E) Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. Tukey HSD test: *p < 0.05.

Journal: bioRxiv

Article Title: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

doi: 10.1101/2023.07.11.548318

Figure Lengend Snippet: (A) Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. (B) Order of events for the generation of the different cell lines. (C) Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. (D) Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. (E) Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. Tukey HSD test: *p < 0.05.

Article Snippet: In order to incorporate mRuby2, we replaced mCherry2 in the donor plasmid (Addgene #121180).

Techniques: CRISPR, Western Blot, Biomarker Discovery, Fluorescence, DNA Methylation Assay, Liquid Chromatography with Mass Spectroscopy